CHANGES IN INTRACELLULAR POTENTIALS AND ION FLUXES OF NEURONS OF MOLLUSKS BY EXTRACELLULAR AND INTRACELLULAR ACTION OF SEVOFLURANE AND DESFLURANE

Intercellular potentials changes of identifiable intact neurons ini solated CNS of great ramshorn (Planorbarius corneus) have been investigated with the help of intercellular microelectrodes and ion currents of isolated neurons of great ramshorns and pond snails (Lymnaea stagnalis) with the fixation of potential influenced by desflurane in concentrations of 1 and 10 mM (1.2 и 12 MAC) and sevoflurane in concentrations of 1 and 5 mM (2.27 and 11.36 MAC). In 3-5 minutes after the start of anesthetics' action some neurons developed insignificant hyperpolarization (for 2-5 mV), and some other developed depolarization (up to 10 mV). When anesthetics were being washed out for 5-10 minutes the reversible depolarization appeared. Changes in ion currents under the influence of anesthetics were more homogeneous compared to changes in biopotentials. Desflurane and sevoflurane in 1 mM concentrations suppressed amplitudes of sodium, calcium and potassium currents by 40%, and inactivation of potassium low currents was speed up. Anesthetics in the concentration of 5-10 mM suppressed currents by 70-80%. It developed fast (for 20-30 seconds) and after this action the amplitudes restored slowly (for 5-15 minutes) and it failed to reach the initial values in all the times. Intercellular activity of sevoflurane did not suppress ion currents, i.e. it was ineffective.

1. Vislobokov A.I., Ignatov Yu.D., Galenko¬Yaroshevsky P.A. et al. Membranotropnoye deistviye pharmakologicheskikh sredstv. [Membrane¬acting action of pharmaceutical substances]. SPb., Krasnodar, Prosvescheniye Yug Publ., 2010, 528 p.

2. Vislobokov A.I., Ignatov Yu.D., Seredenin S.B. Changes of neuron electric activity under aphobazolum action. Eksper. i klin. farm. 2012, vol. 75, no. 6, pp. 3¬7. (In Russ.)

3. Vislobokov A.I., Shabanov P.D. Kletochnye i molekulyarnye mekhanizmy deistviya lekarstv. Seriya: Tsitopharmakologiya. [Cellular and molecular mechanisms of drug actions. Series: Cytopharmacology]. vol. 2, St. Petersburg, Inform¬Navigator Publ., 624 p.

4. Likhvantsev V.V., Ilyin Yu.V., Shmeleva E.A. et al. Impact of anesthesia method on beginning and progression of consciousness disorders in postoperative period in patients with cerebral vascular insufficiency. Vestnik Anasteziol. i Reanimatol., no. 6, 2014, pp. 5¬15. (In Russ.)

5. Tolkunov Yu.A., Sibarov D.A., Frolov D.S. Action of primary afferent neurons of the small bowel is modulated by HNP¬1 defensin by the histamine action. Sensorn. Sist., 2009, vol. 23, no. 1, pp. 79¬86. (In Russ.)

6. Hamakawa T., Feng Z.P., Grigoriv N. et al. Sevoflurane induced suppression of inhibitory synaptic transmission between soma¬soma paired Lymnaea neurons. J. Neurophysiol. 1999, vol. 82, no. 5. pp. 2812¬2819.

7. Hemmings H.C. Neuroprotection by Na+ channel blockade. J. Neurosurg. Anesthesiol. 2004, vol. 16. pp. 100¬101.

8. Hemmings H.C. Sodium channels and the synaptic mechanisms of inhaled anaesthetics. British. J. Anaesthesia. 2009, vol. 103, no. 1, pp. 61¬69.

9. Hirota K., Fudjimura J., Wakasugi M., Ito Y. Isoflurane and sevoflurane modulate inactivation kinetics of Ca2+ currents in single bullfrog atrial miocytes. Anestesiol. 1996, vol. 84, no. 2, pp. 377¬383.

10. Hirota K., Roth S.H. The effects of sevoflurane on population spikes in CA1 and dentate gyrus of the rat hippocampus in vitro. Anesth. Analg. 1997, vol. 85, pp. 426¬430.

11. Kamatchi G.L., Chan C.K., Snutch T. et al. Volatile anesthetic inhibition of neuronal Ca+ channel currents expressed in Xenopus oocytes. Brain Res. 1999, vol. 831, pp. 85¬96.

12. Landoni G., Biondi¬Zoccai G.G.L., Zangrillo A. et al. Desflurane and sevoflurane in cardiac surgery: a meta¬analysis of randomized clinical trials. J. Cardiothoracic and Vascular Anesthesia. 2007, vol. 21, no. 4, pp. 502¬511.

13. Landoni G., Fochi O., Tritapepe L. et al. Cardiac protection by volatile anesthetics. Minerva Anestesiol. 2009, vol. 75, no. 5, pp. 269¬273.

14. Namba T., Ishii T.M., Ikeda M. et al. Inhibition of the human intermediate conductance Ca2+¬activated K+ channel, hIK1, by volatile anesthetics. Eur. J. Pharmacol. 2000, vol. 395, no. 2, pp. 95¬101.

15. Ouyang W., Herold K.F., Hemmings H.C. Comparative effects of halogenated inhaled anesthetics on voltage¬gated Na+ channel function. Anesthesiol. 2009, vol. 110, no. 3, pp. 582¬590.

16. R Core Team. R: A language and environment for statistical computing. R foundation for statistical computing, Vienna, Austria. 2014, URL http://www.R-project.org/.

17. Weigt H.U., Kwok W.M., Rehmert G.C. et al. Voltagedependent effects of volatile anesthetics on cardiac sodium current. Anesth. Analg. 1997, vol. 84, pp. 285¬293.

18. Wu X., Lu Y., Dong Y. et al. The inhalation anesthetic isoflurane increases levels of proinflammatory TNF¬α, IL¬6, and IL¬1β. Neurobiol Aging. 2012, vol. 33, pp. 1364¬1378.

19. Wulf H., Ledowski T., Linstedt U. et al. Neuromuscular blocking effects of rocuronium during desflurane, isoflurane, and sevoflurane anaesthesia. Can. J. Anaesth. 1998, vol. 45, pp. 526¬532.

20. Young C.J., Apfelbaum J.L. Inhalational anesthetics: desflurane and sevoflurane. J. Clinic. Anesth. 1995, vol. 7, pp. 564¬577.